PENGARUH PEMBERIAN KOPI TERHADAP MOTILITAS SPERMATOZOA TIKUS WISTAR YANG DIPAPAR SINAR ULTRAVIOLET

Abstract

Background: Ultraviolet is one of free radical cause factor that can interfere sperm motility. The caffeine present in coffee increases cAMP production that stimulates spermatozoa movement. Chlorogenic acid in coffee acts as a potential source of antioxidant. Objective: This experimental research aims to validate the effect of coffee intake and exposure of Ultraviolet radiation on male wistar rats' sperm motility. Method: Random sampling and posttest only control group design were adapted for this study. A sample of 28 wistar rats were chosen and divided into 4 groups, Control -/+ and Group P1/P2. All samples are given standard meals. Control (-) negative was the baseline ; Control (+) positive was exposed to ultraviolet radiation only ; Group P1 was exposed to ultraviolet radiation and coffee consumption of 180mg/3ml/day ; Group P2 was exposed to ultraviolet radiation and coffee consumption of 360mg/3ml/day. This experimental research was carried out for a period of 30 days. On day 31, all samples were terminated and its sperm motility were examined. Result: Mean of sperm motility in sample groups post intervention : group K(-) = 32,5 ; group K (+) = 11,67 ; group P1 = 40 ; group P2 = 51,67. Oneway ANOVA test showed statistically significant difference in sperm motility in all sample groups. Post Hoc test showed significant difference between experimental Group K(-) and K (+) (p=0,002), Group K(+) and P1 (p=0,000), Group K(+) and P2 (p=0,000). Otherwise, there was no significant effect among Group P1 and P2. Conclusion: The administration of coffee in experimented wistar rats topped with exertion of Ultraviolet radiation promotes significant improvement in sperm motility. Keywords: Ultraviolet, coffee, sperm motility, caffeine, chlorogenic acid