PENGARUH PEMBERIAN EKSTRAK JINTAN HITAM (Nigella sativa) TERHADAP KADAR HEMOGLOBIN TIKUS SPRAGUE DAWLEY SETELAH DIBERIKAN PAPARAN ASAP ROKOK

Abstract

Background : Hemoglobin (Hb) is a heme protein that carries the O2 and CO2 in our body. Cigarette smoking is a factor that makes the Hb level decreses. There are several dangerous substances such as nitric oxide, tar, nicotine and lead inside of it. The black cumin (Nigella sativa) is a herb that contains many active compounds, vitamines and minerals such as thymoquinone, Fe, Vit. C, etc. Due to those reasons, I suspected the black cumin extract would enhance the Hb level. Objective : This study was to determine the effect of black cumin extract in the Sprague Dawley’s Hb level after being exposed by the cigarette smoking. Methods : This study used the post test only control group as its design. It was conducted on 18 Sprague Dawley rats that were devided into three groups: K0 group was the negative control group (no treatment); K1 group was the positive control group (exposed by the cigarette smoking, 4 cigarettes/day); K2 group was the treatment group (exposed by the cigarette smoking, 4 cigarettes/day and also 500 mg black cumin extract perday). After 28 days of study, the rat’s blood was taken in order to count the Hb level. The data was analyzed with One-way ANOVA. Result : Mean Hb level of: K0 group is 14,73 ± 0,56 g/dL; K1 group is 13,56 ± 0,35g/dL; K2 group is 14,96 ± 0,31 g/dL. The Hb mean level of K2 group has significantly increased compared to the K1 group (p = 0,00). Conlcusion : The black cumin extract improved the Sprague Dawley’s Hb level after being exposed by the cigarette smoking. Keywords : Black cumin, hemoglobin, cigarette smoke.