HISTOPATOLOGI SPERMATOGENESIS TESTIS TIKUS WISTAR DIABETES MELITUS

Abstract

Background: In patients with diabetes mellitus, high blood sugar levels lead increasing Reactive Oxygen Spescies resulting in oxidative stress. Subsequently disrupt the process of cell respiration, causing the loss of potential function in mitochondrial membrane and trigger cell apoptosis. Oxidative stress in the testis can impair the process of spermatogenesis in the seminiferous tubules. Objective: To prove the significance of the disruption of the spermatogenesis in male Wistar rats with diabetes mellitus compared to normal Wistar rats. Methods: This study used a true experimental design with post-test only control group design. Total sample of 32 Wistar rats were divided into 2 groups. The control group (K) was only given a standard feed while the treatment group (P) was made diabetic with alloxan 150mg / kg on the 8th day. Each group’s blood sugar levels were measure 3 times. After 21 days the mice wer terminated. Testes were taken for histopathological observation with HE staining and assessed according to the criteria Johnsen score. Results: The median value of the treatment group was 8.6 (range 6 – 9.2), control group of 10 (range 7 – 10). Normality data analysis Shapiro-Wilk test, showed abnormality (p<0.001). The results of non-parametric Mann Whitney differential test showed significant difference between control and treatment groups (p=0.00011). Conclusion: High blood sugar levels in diabetes mellitus conditions can interfere with the process of spermatogenesis. Keywords: Diabetes mellitus, ROS, spermatogenesis, seminiferous tubules.