PENGARUH PEMBERIAN MELATONIN TERHADAP JUMLAH LEUKOSIT PADA TIKUS WISTAR MODEL SEPSIS

Riani, Rizqi Indah and Wicaksono, Satrio Adi (2016) PENGARUH PEMBERIAN MELATONIN TERHADAP JUMLAH LEUKOSIT PADA TIKUS WISTAR MODEL SEPSIS. Undergraduate thesis, Diponegoro University.

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Abstract

Background : Melatonin was a free radical frequently used as an antioxidant. Melatonin played a role in increasing immune response, and supporting cytoprotective process. On some animal models, melatonin has been identified to be able to resist bacteria infections, viruses, and parasites through some mechanisms such as immunomodulation or antioxidant activities. Melatonin could decrease the level of inflammation cytokine, oxidative stress, and mitochondria dysfunction. Melatonin was one of medicine developed as a sepsis therapy. Objective : this research was to find out the melatonin influence on the amount of white blood cells of a wistar rat sepsis model and to obtain the information that melatonin could decrease the number of white blood cells. Methods : this research was an experimental research with a randomized control group using pre and post test. The samples were 12 male wistar rats with certain criteria divided into 2 groups. The first group was given an intraperitoneal injection of lipopolysaccharide (LPS) and was not given melatonin as control group. The second group was given an intraperitoneal injection of lipopolysaccharide (LPS) and was given melatonin by oral sonde as treatment group. After a week, in the eighth day, the blood of each rat was taken from the retro-orbital blood vessel. The statistical test used paired t-test, independent t-test, and Mann Whitney test. Results : In the independent test, the average score of the amount of white blood cells from control group was higher than the experimental group. In the paired t-test, the control group underwent a significant change (p<0,05) compared to experimental group which showed a meaningless result. In the Mann Whitney test, the result of pre-post 1 and post 2 from the control group got a significant increase while the result of pre LPS – post 1 and post 2 from the experimental group got a significant decrease (p<0,05). Conclusion : The melatonin treatment did not cause a significant decrease of the amount of white blood cells. Keywords : Sepsis,The amount of white blood cells, Melatonin, Lipopolysaccharide.

Item Type:Thesis (Undergraduate)
Subjects:R Medicine > RM Therapeutics. Pharmacology
Divisions:Faculty of Medicine > Department of Medicine
Faculty of Medicine > Department of Medicine
ID Code:50780
Deposited By:Ms Lutfiatun Heni
Deposited On:15 Nov 2016 11:06
Last Modified:15 Nov 2016 11:06

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