PERBEDAAN KADAR MALONDIALDEHIDA PADASUBYEKBUKAN PEROKOK, PEROKOK RINGAN DAN SEDANG-BERAT

Abstract

Background : Smoking is a serious problem among teenagers. Smokers can be classified into severalgroups according to its intensity;non-smokers,light smokers, and moderate-to-heavy smokers. Smoking can induce the increase of oxidative stress through carcinogenic substances, free radicals and ROS which exists on the gas and particulate phase of the cigarette smoke. MDA is an oxidative stress biomarker which is easilymeasurable and represents the level of oxidative stress caused by smoking. Aim : To analyzethe difference of serum MDA levels on non-smokers, light smokers and moderate-to-heavy smokers. Methods : Analytical-descriptive study with cross-sectional design. Thirty-six Diponegoro University students were classified into three groups according to Sitepoe’s classification of smoking intensity: non-smokers, light smokers and moderate-to-heavy smokers. Serum MDA levels were measured spectrophotometrically using TBARS method. Statistical analysis were done with One Way ANOVA and Post-Hoc Bonferronitests. Results : Average serum MDA level for non-smokers were11,46 ±0,393 nmol/mL.Average MDA level for light smokers were 11,57 ± 0,948 nmol/mL, and the average MDA levels for heavy smokers were12,76 ± 1,18 nmol/mL. Post Hoc Bonferronitests shown that there is a significant difference between serum MDA levels between non-smokers compared to moderate-to-heavy smokers(p=0,006)and also between light smokers compared to moderate-to-heavy smokers. There is no significant difference(p=1,000)between non-smokers and light smokers. Conclusions : There is a significant serum MDA levels differencebetween non-smokers compared to moderate-to-heavy smokersand also between light smokers compared to moderate-to-heavy smokers. There is no significant difference between non-smokers and light smokers. Keywords: Smoking, oxidative stress, MDA