PENGARUH PEMBERIAN EKSTRAK JINTAN HITAM (NIGELLA SATIVA) TERHADAP KADAR SUPEROXIDE DISMUTASE (SOD) PLASMA PADA TIKUS SPRAGUE DAWLEY YANG TERPAPAR ASAP ROKOK

Abstract

Background: Indonesia is one of the country which smokers is quite high in the world. It is known that cigarette smoke can lower the levels of antioxidants in the body, for instance SOD, thus it could increasing the risk of degenerative diseases. Black cumin is a herb that is known to have effects of natural exogenous antioxidant, thimoquione, that can improve antioxidants in the body. Objective: To analyze the effect of black cumin extract on plasma levels of superoxide dismutase in Sprague Dawley rats that were exposed to smoke. Methods: A true experimental design with post test only control group design study using Sprague Dawley as animal model. After adapted for 7 days, 18 samples were randomly divided into three groups (n=6 pergroup). K1 (negative control), K2 (positive control) was given exposure to cigarette smoke 4 cigarettes / day, P was given exposure to cigarette smoke and black cumin extract 2g / KgBW / day. Examination SOD was measured using ELISA kit on the day 29th, using blood sample from retroorbita vein. Statistical test using Kruskal Wallis. Results: The mean ± SD SOD each group were K1 = 6.34 ± 3.64, K2 = 3.43± 0,34, P = 5.08 ± 2.92, there were no significant differences between groups. (P = 0,208) Conclusion: Black cumin seed extract increases plasma SOD levels in Sprague Dawley rats by exposure to cigarette smoke and black cumin seed extract. Keywords: Black cumin extract,cigarette smoke, plasma SOD levels