PENGARUH MADU TERHADAP GAMBARAN MIKROSKOPIS TESTIS PADA TIKUS WISTAR YANG DIINDUKSI MONOSODIUM GLUTAMAT

Abstract

Background: Monosodium glutamate (MSG) is a food seasoning that is often consumed excessively although it is harmful to the body, including on testis. The harmful effect of MSG on testis must be highlighted because it can lead to infertility. Honey as an antioxidant can counteract the harmful effects of MSG on testis. Aim: To prove the effect of multi level doses of honey on microscopic structure of testis in wistar rats induced to monosodium glutamate. Methods: True experimental study "post test only control group design" using 18 wistar rats were divided into 3 groups, P1 was administered MSG 6 mg/g BW/day, P2 was adminstered MSG 6 mg/g BW/day then honey 2 g/200 g BW/day, P3 was adminstered MSG 6 mg/g BW/day then honey 4 g/200 g BW/day. The treatment was 30 days, then the rats were terminated and the microscopic structure of testis was observed with Johnsen Criteria. Results: P1 showed histological structural damage of testis and 80% of seminiferous tubules were in the category of early maturity arrest. P2 showed the repairment of histology structure of testis compared to P1 and 73.33% seminiferous tubules were in the category of late maturity arrest. P3 showed better histological structure of testis compared to others and 53.33% of the seminiferous tubules in the category of obstructive cases/normal. Hypothesis test showed significant difference between each group ( p<0.05). Conclusions: Administration of multi level doses of honey give protective effect to the histological structure of testis which was exposed to MSG. Keywrods: MSG, honey, microscopic structure of testis.