PENGARUH PEMBERIAN MINYAK Nigella sativa DAN KOMBINASINYA DENGAN SEFTRIAKSON TERHADAP JUMLAH KUMAN Methicillin Resistant Staphylococcus aureus (MRSA) PADA KULTUR OTAK MENCIT BABL/c

Abstract

Background Methicillin Resistant Staphylococcus aureus (MRSA) infection cases reported to be high. This matter is caused by the bacteria resistance to many antibiotics, from this point WHO suggested to looking for new antibiotics, in this research utilize N.sativa oil that have antibacterial and immunomodulator effects. The combination of N.sativa oil with ceftriaxone is expected to resolve MRSA infection cases. Aims To investigate the effect of N.sativa oil administration and its combination with ceftriaxone toward MRSA bacteria count in cultured brain of BALB/c mice. Methods Experimental laboratory with post test only control group design. Total sample were 20 male BALB/c mice randomized into 4 groups and adapted for 7days. Every group was injected with MRSA ATCC 43300 0,2ml, after 16 hours K was given aquabidest 0,03 ml, P1 was given ceftriaxone 0,03 ml, P2 was given N.sativa oil 0,3 ml, P3 was given ceftriaxone 0,03 ml and N.sativa 0,3 ml, after 8 hours would be terminated. Data normality test used Saphiro-wilk test followed by Kruskal-Wallis and Mann-Whitney test. Results The result of the average number of MRSA on brain culture, K= 128x103 157,25x103 cfu/ml; P1= 4,18x1033,22x103 cfu/ml; P2= 0,12x1030,11x103 cfu/ml; P3= 0,68x1031,30x103 cfu/ml. Significant differences was found between K with P2 (p=0,009) and P3(p=0,028), while with P1(p=0,346) wasn’t. Bacteria count between P1 with P2(p=0,009) and P3(p=0,028) showed a significant differences, and between P2 with P3(p=0,754) there was no significant difference. Conclusion Administration of Nigella sativa oil and its combination with ceftriaxone can decrease number of MRSA bacteria in BALB/c mice’s brain cultures. Keywords : MRSA, ceftriaxone, N.sativa oil , in vivo, bacteria count