PENGARUH PAPARAN INSEKTISIDA PADA TESTIS TIKUS SPRAGUE DAWLEY: KAJIAN RISIKO KEGANASAN SEL GERMINAL

Abstract

Background: Insecticides, such as insecticide repellents, contain compounds that are classified as Endocrine Disrupting Chemicals (EDCs). EDCs act as estrogen-like hormones. These compounds can bind to estrogen receptors located in testicular germ cells and mediate carcinogenesis through DNA oxidative damage. Objective: To determine the effect of insecticides exposure on testicular germ cell Methods: This true experiment research design with parallel post test only control group design using Sprague Dawley rat strain 3 days post-natal age (n=25) which were randomly divided into 5 groups (control group (n=5), 25 mg β estradiol 3-benzoate (n=5), burn mosquito repellent (n=5), 3 ml of liquid mosquito repellent (n=5), and 4 ml of liquid mosquito repellent (n=5)). After 100 days, testicular was taken and examined its germ cell malignancies histopathologically using HE staining. Kruskal-Wallis and continued using Mann-Whitney statistical analysis were done to seek the difference between control and treatment groups. Results: There was an enhancement in the number of testicular germ cells that lead to malignancy in each treatment group compared to those of control group. There were significant differences between treatment groups 25 mg β estradiol 3-benzoate (p=0.008), burning mosquito repellent (p=0.019), and liquid repellent 4 ml (p=0.025) compared to those of control group. However, the difference was found not significant between 3 ml liquid repellent (p=0,589) compared to those of control group. Conclusions: Exposure to insecticides may result in significant changes in testicular germ cell histology that leads to malignancy. Keywords: Insecticides, testicular germ cell malignancy, Endocrine Disrupting Chemicals. 1 Undergraduate Student, Faculty of Medicine Diponegoro University 2 Staff of Anatomy Department, Faculty of Medicine Diponegoro University 3 Staff of Anatomical Pathology Department, Faculty of Medicine Diponegoro University