COMPARATIVE EFFECTIVENESS AND OPTIONAL PERIOD OF THE FLOTATION METHOD USING NaCl, ZnSO4 AND MgSO4 FOR THE DIAGNOSTIC OF SOIL-TRANSMITTED HELMINTHS

Abstract

Background. Factors that affect the effectiveness of flotation method in Soil-transmitted helminths diagnostic are flotation solution and optional period. Further research is required to determine the optional period that gives the best effectiveness from three flotation solutions, which are NaCl, MgSO4, and ZnSO4. Aim. To compare the effectiveness of NaCl, MgSO4 and ZnSO4 as flotation solution in flotation method and the best optional period of each floating solution determined by counting number of eggs. Methods. This research was quasi-experimental analytic with two independent variable. Independent variables 1 were flotation solutions (saturated NaCl, MgSO4 and ZnSO4) . Independent variables 2 were optional period (15,30,45,60 and 70 minutes). Each combination of treatments performed on 8 samples. Results. There were significant differences between different of optional periods in one solution .The same optional period for each solution ( saturated NaCl, saturated MgSO4 and saturated ZnSO4) were not gives siginificant differences. The difference considered significant when the p value < 0.05. The most number of eggs found in NaCl was at 60 minutes, ZnSO4 at 45 minutes, and MgSO4 at 45 minutes). Conclusion. The optimum of optional period for each solution were: saturated NaCl (60 minutes), ZnSO4 (45 minutes), and MgSO4 (45 minutes). Flotation method using ZnSO4 as solution with 45 minutes as optional period considered as the most effective method based on number eggs found and time. In other hand, the effectiveness of the flotation method obtained either with NaCl, MgSO4 and ZnSO4 in all level of optional period were same. Keywords: flotation method, soil transmitted helminths, parasite diagnostic