Production of Active Compounds By Plant Mesophyll Cells Suspension Culture

Abstract

Extraction process to obtain active compounds from plants need lots of plants and should be available continuously. Efforts to address sustainability of materials for the supply of active compounds is by culture in vitro, one of which is by cell suspension cultures. With this culture can produce the active compound is needed and with good engineering in the engineering, media arid and the environment can produce a higher compound concentration. Centella asiatica L. Urban is a medicinal plant that is widely used in herbal medicine industry in Indonesia. Cell suspension culture of mesophyll of Centella asiatica L. Urban leaves were carried out in three steps: isolation, purification and cell culture. The aim of this research is to invetigate the influences of leaves position (age of leaves) and concentration of macerozyme. Data collected were analyzed using Anova and Duncan's test at 0.005. Sucrose was added in the production medium at the concentration of 0.0-2.5-5.0 and 7.5 percent respectively. Qualitation analysis of asiaticoside produced by cell biomass were carried out by using thin layer chromatography. The result revealed that mesophyll cell could be used as explant for suspension culture. The highest biomass produced with highest viability were found in cell isolated from second leaves treated with 0,1% macerozyme (1.49.10 7 cell/ml). In the case of suspension culture, addition of sucrose to the production medium gave pcv 8.5%, 2,5% sucrose pcv 22%. Sucrose 5% pcv 21,5% and 7,5% pcv 15,75%. The asiaticoside production not only depended on sucrose alone, because in 0% sucrose it was detected too.