PENGARUH PEMBERIAN FOLAT TERHADAP KADAR HOMOSISTEIN DAN PROFIL LIPID PADA TIKUS DIABETES

Abstract

Background: DM is highly increasing and becomes the main health problem in the world. DM is related to the homocysteine (Hcy) elevation and the occurence of dislipidemia. Folate takes a role as methyl donor for Hcy metabolism to become methionin. The process of Hcy remethilation does not only need folate but also coline, betain, and serin. Objective: To prove the influence of 30 day-leveling-dosage folate supplementation to Hcy and lipid profile levels on SD rats which are STZ induced. Method: An experimental study with a randomized controlled group pretestpostest design. Subject population was 30 SD rats induced with STZ 40 mg/kgbb i.p, devided into 5 groups . Hcy serum was measured with ELISA, and profile lipid by method of CHOD-PAP. The data were analized with wilcoxom test and coupled t-test. Result: The elevation of glucose level at group X1:207 mh/dl, X2: 5 mg/dl, X3: 109 mg/dl. No significant difference of Hcy level at 2 ppm and 4 ppm. Significant difference of Hcy level of 8 ppm folate (p=0,043). No significant difference of trigliseride, cholesterol, LDL level at each group. Significant difference of HDL level at 2 ppm folate (p=0,04); this, perhaps, because of the glucose high elevation. Cholesterol level decreased at each level. Trigliseride and LDL levels decreased at 4 ppm folate. Conclusion: Folate dosage supplementation (2 ppm,4 ppm,8 ppm) to DM rats is able to decrease Hcy and cholesterol levels. Folate unstraightly influence lipid profile, and control on glucose level is needed. Keywords: folate, homocystein, lipid profile,rats, DM