Pengaruh Pemberian Jus Mengkudu (Morinda Citrifolia L) Terhadap Nitric Oxide (NO) Dan Reactive Oxygen Intermediate (ROI) Makrofag Tikus Yang Terpapar Asap Rokok The Effect Of Noni Juice (Morinda Citrifolia L) On Nitric Oxide (NO) And Reactive Oxygen Intermediate (ROI) Macrophages On Mice Exposed To Cigarette Smoke

Abstract

Background : Morinda citrifolia (mengkudu) has been acknowledged for its antioxidant content that can lower the oxidant measured based on the production of NO and ROI macrofag. Purpose : To know the difference on the production of bronchoalveolar NO and ROI macrophages of mice exposed to the cigarette smoke with and without given mengkudu juice. Method : This is a laboratorial experiment with the post test only control group design using 20 wistar mice divided randomly into 4 groups being exposed to cigarette smoke twice a day. Control group: not given any treatment. P1, P2, P3 group each had given mengkudu juice with the dosage of 1 ml per day, 2 ml per day, 4 ml per day for 30 days. Bronchoalveoli macrophages is taken to be examined on the production of NO and ROI. The ROI macrophages production data are analyzed by using one way Anova test. The ROI macrophages production data are analyzed by using Kruskal Wallis test. Result : One way Anova test shows that there is no significant difference on NO macrophages production with p= 0,079. Kruskal Wallis test shows that there is no significant difference on ROI macrophages production index with p= 0,135. Conclusion : The distribution of mengkudu juice (TNJ) results in a statistically non significant difference on ROI macrophages on mice exposed to cigarette smoke. Key words : Mengkudu juice (Morinda citrifolia), cigarette smoke, NO, ROI