Determination Hypoiodous Acid (HIO) By Peroxidase System Using Peroxidase Enzyme

AL-BAARRI, Ahmad Ni’matullah and LEGOWO, Anang Mohamad and WIDAYAT, Widayat and ABDUH, Setya Budi M and HADIPERNATA, M. and WISNUBROTO, Wisnubroto and ARDIANTI, D.K. and SUSANTO, M.N. and YUSUF, M. and DEMASTA, E.K. (2017) Determination Hypoiodous Acid (HIO) By Peroxidase System Using Peroxidase Enzyme. In: 3rd International Conference on Tropical and Coastal Region Eco Development 2017. (Submitted)

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Abstract

It has been understood that peroxidase enzyme including peroxidase serves as catalyzer to enzymatic reaction among hydrogen peroxide and halides, therefore this research was done for generating hypoiodous acid (HIO) from peroxidase system using peroxidase enzyme. Hydrogen peroxide, potassium iodide, and peroxidase enzyme were used to produce HIO. Determination the amount of formed HIO was done using 2,2'-azino-bis(3- ethylbenzothiazoline-6-sulphonic acid) or ABTS as substrate through the colorimetric measurement of hydrogen peroxide residue during reaction process using at 412 nm. The result indicated that residual hydrogen peroxide showed the minimum concentration after 60 minutes reaction time. Because the reaction started at the beginning time of mixing, hydrogen peroxide was unable to be eliminated totally to produce HIO. The reaction of peroxidase system was able to determine the beginning of mixing process but the reaction process could not eliminate the initial concentration of hydrogen peroxide indicating the maximum amount of production of HIO could be determined. In conclusion, the less of H2O2, higher HIO obtained and peroxidase enzymes can accelerate the formation of HIO.

Item Type:Conference or Workshop Item (Paper)
Subjects:T Technology > TP Chemical technology
S Agriculture > SF Animal culture
Divisions:Faculty of Animal and Agricultural Sciences > Department of Food Technology
ID Code:78672
Deposited By:INVALID USER
Deposited On:09 Dec 2019 17:06
Last Modified:20 Jan 2020 11:40

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