Characterization and Identification of Antibacterial Compound from Pseudoalteromonas piscicida Associated with Chromodoris lochi

Abstract

The present study was focused on characterization and analyzed the active compound against human pathogenic bacteria. Thirty-three isolates bacteria associated C. lochi from Saparua Island, were screened as antibacterial activity against strain bacteria of Multi-Drug Resistant (MDR). Screening antibacterial activity was done by the overlay method; the largest clear zone of activity was chosen for further analysis. Identified species of the best candidate was done using molecular work (16s rDNA genes with specific primer). Extraction of bio compound was done with three different solvents (methanol, ethyl acetate, hexane) into paper disk plate assay. The compound was successfully characterized by LCMS/MS spectroscopic data. The isolate SM-N-3-7 showed inhibitory activity against MDR pathogens viz., Klebsiella pneumonia, Bacillus subtilis, Staphylococcus aureus, Micrococcus luteus, Escherichia coli. The fraction active was successfully isolation from the ethyl acetate fraction. The structure compound extracted from SM-N-3-7 was identified as (E)-Hexadecyl-ferulate and Bis (2-ethylhexyl) phthalate. Based on molecular identification, the isolate SM-N-3-7 was closely related to 99% with Pseudolateromonas piscicida. We concluded that P. piscida associated C. lochi has great potential for the antimicrobial agent.