Antibacterial potential of nudibranch-associated bacteria from Saparua and Nusa Laut Islands, Indonesia

Kristiana, Rhesi and Sibero, Mada T. and Farisa, MACELLYNE YOHANNA and Ayuningrum, Diah and Dirgantara, Dio and Hanafi, Muhammad and Radjasa, Ocky Karna and Sabdono, Agus and Trianto, Agus Antibacterial potential of nudibranch-associated bacteria from Saparua and Nusa Laut Islands, Indonesia. BIODIVERSITAS , 20 (7). pp. 1811-1819. ISSN 1412033X

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Official URL: https://smujo.id/biodiv/article/view/3962/3278

Abstract

Infections caused by multidrug-resistant bacteria are the international health issue that triggers the urgency of finding new antibacterial agents. The aim of this study was to obtain the nudibranchs-associated bacteria that have bioactivity against multidrug-resistant bacteria. A total of 13 species of nudibranch were identified based on morphological characterization. Overlay methods were used for the screening of the isolates bioactivity against six pathogenic multidrug-resistant bacteria. The Minimum Inhibition Concentration (MIC) of the crude extract was evaluated against Methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli, Bacillussubtilis, Klebsiella pneumonia, Micrococcus luteus,and Extended Spectrum Beta Lactamases E. coli (ESBL) using MTT method. A total of 145 isolates were obtained which eleven of the isolates showed antibacterial activity against the pathogenic bacteria. The MIC tests showed that the best activity was isolate SM-S-9-15 and SM-N-3-7. The methanolic extract of isolate SM-S-9-15 active to all of the pathogenic bacteria, while the ethyl acetate extract of the isolate SM-N-3-7 active to the E. coli, B. subtilis, K. pneumoniaat the concentration of 500 g/mL. According to 16S ribosomal RNA gene sequence-based identification, all active isolates belong to Virgibacillusmarismortui, V. dokdonensis, Bacilluskochii, Vibrioalgynoliticus,and Pseudoalteromonaspiscicida.

Item Type:Article
Subjects:S Agriculture > SH Aquaculture. Fisheries. Angling
Divisions:Faculty of Fisheries and Marine Sciences > Department of Marine Science
ID Code:76014
Deposited By:Dr. , S.T., M.Sc., Ph.D. Agus Trianto
Deposited On:04 Sep 2019 08:58
Last Modified:04 Sep 2019 08:58

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