The bioactivity of bacterium and fungi living associatewith thespongeRenierasp.against multidrug-resistant Staphylococcus aureusand Escherichia coli

Abstract

The bioactivity of bacterium and fungi living associate with the sponge Renierasp.against multidrug-resistant Staphylococcus aureusand Escherichia coli.Biodiversitas 20:2302-2307.The study aimed to isolate and identify the sponge-associated microorganisms producing the antibacterial substances. The sponge Renierasp. was collected by hand during skin diving in Karimunjawa Islands, Indonesia. The microbial symbionts were isolated with the dilution method and screened with the overlay method against the MDR S. aureusand E. coli. The bacterium was cultured in Zobell medium, while the fungi were cultured in malt extract broth (MEB) medium. The isolates were identified based on the molecular method. A total of 46 bacteria and 43 fungi were isolated, which 7 bacteria and 20 fungi exhibited antibacterial activity against the MDR E. coliand S. aureusstrains.The molecular identification revealed that theactive isolates close toPseudoalteromonas maricaloris(99%), Aspergillus nomius(96%),Eurotium rubrum(99%), and Penicilliumsp. (100%). Fractionation of K.J.16.U extract gave a fraction that active totheS. aureusand E. colistrains at concentration 150 and 15 μg disk-1. The fraction K.J.16.U.1.4.4 exhibited stronger activity than that exhibited by chloramphenicolat 150 μg disk-1. The sponge Renierasp. collected from Karimunjawa Islands comprise bacterial and fungal isolates produced antibacterial compounds that inhibited the growth the MDR E. coliand S. aureusstrains