Pengaruh Pemberian Kapang Chrysonilia Crassa atau Rhizopus Oryzae yang Diisolasi dari Ileum Ayam Kampung terhadap Performa Ayam Broiler yang Tercekam Panas

Abstract

The study aimed to evaluate the antioxidant activity of the fungi C. crassa and R. oryzae as well as to investigate the effect of the fungi on the performances of broiler chickens raised under heat stress. The antioxidant activity of the fungi was assessed in vitro according to 2,2diphenylpicrylhydrazyl (DPPH) and 2,2’-azino-bis(3-ethyl-benzthiazolin-6-sulfonicacid) (ABTS) methods. In the in vivo study, 240 broilers (21 days of age) were distributed to 6 treatment groups with 5 replicates (pen) and 8 birds per pen. These treatments included: chicken reared at 28 ± 2°C (CONT), chickens reared at 35 ± 2°C (HS-CONT), chickens reared at 35 ± 2°C and given anti stress (HS-VIT), chicken reared at 35 ± 2°C and given rice bran containing R. oryzae (HS-RO), chickens reared at 35 ± 2°C and given rice bran containing C. crassa (HS-CC) and chickens reared at 35 ± 2°C and given rice bran (HS-RB). Chickens were provided with commercial diets with or without supplementation according to the treatments. Body weight, feed intake and feed conversion were calculated on day 28 of the experiment. The fungus R. oryzae had antioxidant activity corresponding to vitamin C and was higher than that of C. crassa. Chickens reared at 28°C showed higher and lower growth and feed conversion, respectively, than those of reared at 35°C. Supplementation with the fungi or anti stress could not improve the performance of 28-days old broilers exposed to heat stress. Keywords : antioxidant, broiler chickens, C. crassa, performance, R. oryzae, heat stress