Aktivitas Antagonis Bakteri yang Berasosiasi dengan Teritip (Balanus sp.) terhadap Bakteri Escherichia coli dan Bacillus cereus

Abstract

Marine invertebrates are the most productive source of bioactive compounds. However, most of the compounds originally produced by the microorganisms living associated with the invertebrates. Barnacle is an invertebrate that has a unique interaction with bacteria. Escherichia coli and Bacillus cereus are the cause of the foodborne disease that causes of remarkedly losses in the food industry. The barnacle was collected from Panjang Island, Jepara. The bacterial isolates were obtained by serial dilution followed by isolation based on morphological characteristics. The antagonistic assay to E. coli dan B. cereus was utilized for screened the isolates. Then, the active isolates were cultured for the further test with the disc diffusion agar method of the supernatant followed by the extracts. The most active isolates were identified based on molecular method. A total of 14 isolates were obtained from the Balanus sp., which six of them have activity against the E. coli and B. cereus. The isolates TJ 5.4 and TJ 5.5 have the strongest activity the bacteria. Base on the analyses of the BLAST and phylogenetic tree the isolates showed 99% homology the Bacillus wiedmannii (TJ 5.4) and Lysinibacillus macroides (TJ 5.5).