Human Bone Marrow-Derived Mesenchymal Cell Reactions to 316L Stainless Steel: An in Vitro Study on Cell Viability and Interleukin-6 Expression

Anwar, Iwan Budiwan and Santoso, Asep and SAPUTRA, Eko and ISMAIL, Rifky and Jamari, J. and van der Heide, Emile (2017) Human Bone Marrow-Derived Mesenchymal Cell Reactions to 316L Stainless Steel: An in Vitro Study on Cell Viability and Interleukin-6 Expression. Advanced Pharmaceutical Bulletin, 7 (2). pp. 335-338. ISSN 2228-5881

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Official URL: https://apb.tbzmed.ac.ir/Article/APB_19332_2017042...

Abstract

Purpose: Human bone marrow-derived mesenchymal cell (hBMC) reactions to 316L stainless steel (316L-SS) have never been evaluated. The objective of this study was to assess cell viability and interleukin-6 expression of hBMC cultures upon treatment with a 316L-SS implant. Methods: A cytotoxicity analysis was conducted with a 3-(4,5-dimethylthiazol 2-yl)-2,5-diphenyltetrazolium (MTT) assay after a period of 24, 48 and 72 hours of incubation. Expression of interleukin-6 was measured using enzyme-linked immunosorbent assay (ELISA). Results: Cell viability measurement was performed via IC50 formula. All treatment group showed a > 50 % cell viability with a range of 56,5 - 96,9 % at 24 hours, 51,8-77,3% at 48 hours and 70,1- 120 % at 72 hours. Interleukin-6 expression was downregulated subsequent to treatment with 316L-SS compared to the control group. Conclusion: We found that 316L-SS did not exhibit toxicity towards hBMC culture.

Item Type:Article
Uncontrolled Keywords:316L stainless steel, Human bone marrow-derived mesenchymal cells, Cell viability, Interleukin-6
Subjects:T Technology > TS Manufactures
R Medicine > R Medicine (General)
Divisions:Faculty of Engineering > Department of Mechanical Engineering
Faculty of Engineering > Department of Mechanical Engineering
ID Code:71650
Deposited By:Mr. Sugeng Priyanto
Deposited On:15 Apr 2019 18:45
Last Modified:24 Aug 2019 14:07

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