ISOLATION AND IDENTIFICATION OF PYTHIUM FROM SOIL

Abstract

ABSTRACT The objectives of the experiment were to isolate and identify the Pythium from soil. The experiment used a soil dilution plate and selective medium. The soil was taken from three different areas in Pedurungan district of Semarang City. The first soil was taken from a field planted with Ipomoea aquatica and was fertilized with urea. The second soil was taken from a dry field which was uncultivated, and the third soil was from a rice field which has never been fertilized or treated by any chemical. The key of Robertson (1979) and Plaat-Niterink (1981) were used to identify the Phytium and then two isolates was chosen. The results showed that there were sixteen different isolates from the three different soils. In the first, second and third soils were obtained eleven isolates, two isolates and three isolates, respectively. The identification of two isolates chosen showed that isolate number 2 was determined as Pythium aphanidermatum Fitzpatrick and isolate number 6 was determined as Pythium periplocum Drechsler. These Phytium isolates need further elucidation for any fermentative purpose in processing high cellulosic feedstuffs. Keywords : isolation, identification, Pythium, soil