MOLECULAR CHARACTERIZATION OF Dunaliella salina AND Chlorella vulgaris FUSANT USING 18SrDNA GENE

Kusumaningrum, Hermin Pancasakti and Zainuri, Muhammad (2016) MOLECULAR CHARACTERIZATION OF Dunaliella salina AND Chlorella vulgaris FUSANT USING 18SrDNA GENE. Undip.

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Abstract

Protoplast fusion was found to be an efficient method in improving carotenoid production from fusant of carotenogenic microalgae D. salina and C. vulgaris. Molecular characterization is needed for identifying the dominant parental genome in the fusant using ribosomal DNA sequences. The research was carried out by analyzing the gene encodes for 18S rDNA of fusant and determining relationship of fusant with D. salina and C. vulgaris species from GenBank. Quantitative analysis showed that C. vulgaris was not remarkably dominant in fusant with 84 % similarity compare to D. salina with 82 % similarity. The result indicated that the fusant gained both character from their parents.

Item Type:Other
Subjects:Q Science > QH Natural history > QH301 Biology
Divisions:Faculty of Science and Mathematics > Department of Biology
ID Code:67768
Deposited By:Mr Wondo Perpus
Deposited On:14 Dec 2018 09:41
Last Modified:09 Apr 2019 20:02

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