Potensi Senyawa Bioaktif Karotenoid Silase Ikan dari Berbagai Produk Perikanan Laut Untuk Pakan Akuakultur

Abstract

This study was designed to evaluate the potential of fish silage made from marine materials (shrimp head, blue crab waste, mud crab waste, squid and tigawaja trash fish) as sources of bioactive substances of carotenoid for aquaculture feeds. The raw materials and the fish silage were analysed for the carotenoid contents through an extraction of the materials with tetrahidrofuran and injection by microsyringe using a HPLC (Merk/lmbada/max 461). The fish silage were manufactured by mixing 3% formic acid and stored for 7 days. Carotenoid analysis showed that β-caroteen was the major carotenoid pigment found in all marine materials with tigawaja trash fish had the highest β-caroteen content (57.481 mg/ 100 g) among the others. Shrimp head had the highest aztaxanthin level (22.485 mg/100 g). The manufacture of fish silage, in general, resulted in the decreased carotenoid contents, however some the fish silage had a higher carotenoid content. The aztaxanthin levels in squid increased 240% from the fresh (3.748 mg/100 g) to the silage (33.462 mg/100 g) and the zeaxanthin levels in blue crab waste increased from 26.335 mg/100 g (the fresh) to 33.462 mg/100 g (the silage). The carotenoid source potency of the squid silage was high for aztaxanthin, the blue crab silage was high for zeaxanthin and lutein contents and the tigawaja trash fish was high for -caroteen content. This study indicates that the bioactive substances contents of carotenoid (aztaxanthin) can be significantly improved by the manufacture of fish silage. The fish silage manufactured of squid, blue crab waste, and trashfish tigawaja were found to be the most potential carotenoid source for aztaxanthin, zeaxanthin, and -caroteen, respectively in formulated diets for aquaculture.