The Intracellular Cryoprotectant Effects in Preserving Goramy Spermatozoa after Two Days Sub-Zero Freezing

Abinawanto, Abinawanto and Fitrianingrum, Nisa and LESTARI, RETNO and Sudaryono, Agung and Rostika, Rita and Fujaya, Yushinta (2015) The Intracellular Cryoprotectant Effects in Preserving Goramy Spermatozoa after Two Days Sub-Zero Freezing. Aquacultura Indonesiana, 16 (1). pp. 16-21. ISSN 2477 - 6939

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Abstract

The spermatozoa quality of goramy after 2 d sub-zero freezing was examined. The quality of spermatozoa examined included motility, viability, and abnormality. We aimed to determine the optimum concentration of glycerol protecting spermatozoa during preservation. We used 0%, 1%, 3%, 5%, 7%, and 9% of glycerol, respectively. Sperms were diluted by the combination of glycerol and fish ringer (1 part of sperm + 3 part of solvent). The dilute sperms were then equiliberated at 4°C for 45 min, and were freezed at -34°C for 2 d. Thawing was then carried out at 30°C for 2 min. Based on Dunnet test, 5% of glycerol was the optimum concentration maintaining spermatozoa motility (75.95±4.76)%.

Item Type:Article
Uncontrolled Keywords:Glycerol; Osphronemus goramy; Spermatozoa motility; Sub-zero freezing; Viability and Abnormality
Subjects:S Agriculture > SH Aquaculture. Fisheries. Angling
Divisions:Faculty of Fisheries and Marine Sciences > Department of Fisheries
ID Code:67537
Deposited By:Mr. Sugeng Priyanto
Deposited On:05 Dec 2018 05:57
Last Modified:07 Dec 2018 18:16

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