Antibacterial activity of mangrove Avicennia marina leaves extract against Virgibacillus marismortui and Micrococcus luteus bacteria

Abstract

Sources of bioactive compounds are widely found in natural resources such as mangrove plants. Avicennia marina is one of the natural materials that contain antibacterial bioactive compound. The purpose of this research is to investigate the potency of A. marina leaves as a source of antibacterial agents against Virgibacillus marismortui and Micrococcus luteus. Leaves of A. marina were collected from mangrove ecosystem Tugurejo village, Semarang. Experimental laboratories method was used in this research, while data was analyzed descriptively. Sample was extracted by using methanol. Agar Diffusion method was utilized on anti bacterial test against V. marismortui and M. luteus with concentration of 1000 μg disc⁻¹, 500 μg disc⁻¹ and 250 μg disc⁻¹. The result of the test showed that A. marina leaves extract of 1000 μg disc⁻¹ could inhibit both bacteria, with 13.50±0.13 mm inhibition zone for V. marismortui and 13.46±0.32 mm for M. luteus, at concentration of 500 μg disc⁻¹ to inhibit both bacteria with inhibition zone of 5.69±0.30 mm for V. marismortui and 6.56±0.51 mm for M. luteus, the concentration of 250 μg disc⁻¹ did not inhibited bacteria. Antibiotic Amoxicillin as positive control showed smaller inhibition zone compared to inhibition zone that was formed by the extract of mangrove leaves. It was assumed because the high concentration of mangrove extracts, so that the bigger inhibition zone could be formed compared to the antibiotic treatment.