Exploration of Sea Cucumbers Stichopus hermanii from Karimunjawa Islands as Production of Marine Biological Resources

Pringgenies, Delianis and Rudiyanti, Siti and Yudiati, Ervia (2018) Exploration of Sea Cucumbers Stichopus hermanii from Karimunjawa Islands as Production of Marine Biological Resources. IOP Conference Series: Earth and Environmental Science . pp. 1-8. ISSN 1755-1315

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Official URL: http://iopscience.iop.org/article/10.1088/1755-131...

Abstract

This research aim was to study the potential of Stichopus hermanii to determine the amino acid, chondroitin, and glucosamine contents, to discover its antibacterial and anti-cancer agent. The samples were rinsed prior to separation, with only the corpus being used in the study. Sea cucumber extract was then processed using HPLC to trace contents of amino acid, chondroitin, and glucosamine contents. The samples were then put into test against several strains of pathogenic bacteria by means of diffusion for any biological activity. The anti-cancer test was performed by human ovarian cancer cell line (KOC7C) method. The study showed that the extract of Stichopus hermanii has the potency to inhibit the growth of active ovarian cancer cells. The qualitative test of the sea cucumber extract showed that it is capable of suppressing the growth of several strains of pathogenic bacteria identified as Staphylococcus aureus, Escherichia coli, Vibrio voinivica, and Pseudomonas sp. HPLC results showed that the extract contained amino acid (mg/100g), the highest being Collagen (11200), followed by Glycine (3760), Glutamic Acid (3700), Aspartic Acid (2540), Alanine (2140), Proline (2050), Arginine (2050), Tyrosine (1430), Threonine (1270), Leucine (1170), Valine (1050), Serine (971), Isoleucine (816), Phenylalanine (713), Lysine (639), Methionine (383), Cystine (263) and Histidine (208). The extract also contained Chondroitin Sulfate (4200) and Glucosamine

Item Type:Article
Subjects:Q Science > Q Science (General)
Divisions:Faculty of Fisheries and Marine Sciences > Department of Marine Science
ID Code:64696
Deposited By:Delianis Pringgenies
Deposited On:13 Sep 2018 11:44
Last Modified:12 Jul 2019 20:19

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