Penapisan Bakteri Simbion Gastropoda Stramonita armigera Penghasil Senyawa Antibakteri Multi Drug Resistant dari Perairan Ternate

Pringgenies, Delianis and Dananjoyo, Mijil Ciptaning (2010) Penapisan Bakteri Simbion Gastropoda Stramonita armigera Penghasil Senyawa Antibakteri Multi Drug Resistant dari Perairan Ternate. Jurnal Natur Indonesia, 13 (3). pp. 200-206. ISSN 1410-9379

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Abstract

Antibiotic resistance is an ability of bacteria to hold the antibiotic effect. It was reported that there is a humanpatogen bacteria that resistance to one or more classes of antibiotic. It become a problem on medical world. To solve those problems, it is necessary to search the new antibiotic compounds that more effective and efficient to solve the problem of Multi Drug Resistance (MDR). The secondary metabolite-producing marine invertebrates and symbiont microorganisms, have prospect as an antibiotic. The symbiont microorganisms may produce the secondary metabolite similar to their host. The aims of the reseach were to determinate of gastropods symbiont bacteria that capable of producing Antibacterial MDR (Multi Drugs Resistant) Compound. Sample of Molusc were collected from Ternate (Molucas) islands. Isolation of symbiotic bacteria, screening for bacteria which producing secondary metabolites as anti-MDR bacteria, antibacterial test, isolation of clinical pathogenic bacteria (MDR), conducting anti-bacterial sensitivity test, sensitivity test for antibacterial, DNA exctraction, DNA amplification based on PCR method, DNA sequencing. Result of 16S r-DNA sequence was then analyzed and edited using GENETYX program and followed by 16S rDNA sequence analysis. The result showed that 17 strains were isolated from gastropods Stramonita armigera. Antibacterial assays showed that TSA 8.7 isolate have ability to inhibit Pseudomonas sp., Escherichia coli dan Enterobacter sp. the molecular analyses showed that isolate TSA 8.7 closed by related to Vibrio sp. Strain JZDN1, with 98% of homology. Based on this experimental result, it could be concluded that gastropods-symbiont bacterium Stramonita armigera capable of producing antibacterial compound against strain Multi Drug Resistant (MDR). There is 11 isolates of gastropods-symbiont bacteria Stramonita armigera that have an antibacterial MDR activity.

Item Type:Article
Subjects:Q Science > Q Science (General)
Divisions:Faculty of Fisheries and Marine Sciences > Department of Marine Science
ID Code:64689
Deposited By:Delianis Pringgenies
Deposited On:13 Sep 2018 10:08
Last Modified:12 Jul 2019 19:35

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