ANTIBACTERIAL ACTIVITY FOR MULTI DRUG RESISTANCE (MDR) BACTERIA BYSEA CUCUMBER Stichopus vastus EXTRACT FROM KARIMUNJAWA ISLANDS - INDONESIA

Delianis Pringgenies, DP and Ali Ridlo, AR and Nerva Sembiring, NS (2017) ANTIBACTERIAL ACTIVITY FOR MULTI DRUG RESISTANCE (MDR) BACTERIA BYSEA CUCUMBER Stichopus vastus EXTRACT FROM KARIMUNJAWA ISLANDS - INDONESIA. Jurnal llmu dan Teknologi Kelautan Tropis, 9 (2). pp. 695-707. ISSN 2087 - 9423

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Official URL: http://journal.ipb.ac.id/index.php/jurnalikt/artic...

Abstract

The study aims to explore the antibacterial activity of Stichopus vastus against pathogenic MDR bacteria. Analysis of samples of sea cucumbers included extraction, fractionation, and analysis of bacterial sensitivity test Gas Chromatography-Mass Spectrometry (GC-MS), the extraction process is carried out by solid-liquid extraction method. Fractionation was done with Open-Column Chromatography (OCC). Sensitivity test of bacteria was done using an agar diffusion method according to the Kirby-Bauer (Ref). The study revealed that from 5 species MDR bacteria, which are Coagulant negative stapylococi (CNS), E. coli, Enterobacter 5, Klebsiella sp. dan Pseudomonas sp. There are two MDR bacteria had the most sensitive responses by the extract of sea cucumber, which were Enterobacter-5 and Klebsiella sp. The two bacteria were tested against five bioactive fractions obtained from OCC. Fraction criteria-2 had the highest antibacterial activity against Enterobacter-5 and Klebsiella sp, with serial concentration of 20 μg ∙ disc–1, 40 μg. disc–1 and 80 μg. disc–1. Largest inhibition zone were obtained from 80 μg. disc–1 againts the two bacteria were (14.73±0.48) mm and (11.22±0.85) mm respectively. GC-MS Analysis revealed that fraction criteria-2 had (or consisted of) cyclohexhane, ethanol, butanoic and pentanoic acids. Keywords: antibacterial activity, multi drug resistance (MDR), sea cucumber, Stichopus vastus

Item Type:Article
Subjects:Q Science > Q Science (General)
Divisions:Faculty of Fisheries and Marine Sciences > Department of Marine Science
ID Code:64667
Deposited By:Delianis Pringgenies
Deposited On:13 Sep 2018 04:36
Last Modified:08 Jul 2019 15:23

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