Isolation of Local Lipolytic Isolate from Domestic Compost

Syihab, Syifa F. and Madayanti, Fida and Akhmaloka, A. (2015) Isolation of Local Lipolytic Isolate from Domestic Compost. In: The 5th International Seminar on New Paradigm and Innovation on Natural Sciences and Its Application (5th ISNPINSA) i , 7-8 Oktober 2015, Semarang.

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Abstract

Screening of lipolytic bacteria from domestic compost resulting an isolate namely AL17. Morphological analysis shows that the isolates were rod shape and belong to negative gram bacteria. The 16S rRNAs genes of the bacteria have been sequenced, and phylogenetic analysis showed that the isolates were close to genus Pseudoxanthomonas. The enzyme production was synchronized with bacterial growth and reached a maximum level during the late-stationary phase. The optimum pH and temperature of enzyme activity were at pH 9.0 and 60°C.The isolate also showed alcohol tolerance in medium containing 3% and 5% methanol. The ability of bacterial cells to tolerate methanol is an important cell characteristic that determines their use as a biocatalyst in transesterification and other industrial process.

Item Type:Conference or Workshop Item (Paper)
Subjects:Q Science > Q Science (General)
Divisions:Faculty of Science and Mathematics > Department of Chemistry
ID Code:62530
Deposited By:Faculty of Engineering
Deposited On:25 May 2018 15:06
Last Modified:25 May 2018 15:06

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