Histological Structure of Mice (Mus Musculus L.) Liver after Administration of Ethanol Extract and Spinasterol from Senggugu (Clerodendron Serratum L) Leaves

Abstract

Senggugu (Clerodendron serratum) is a plant that used as traditional medicine. Additionally,C. serratum contains several compounds that are classified into natural antifertility products, namely: apigenin, alkaloids, flavonoids, steroids, and triterpenes. This study was conducted to investigate the toxic effect of ethanol extract and spinasterol from C. serratum L. leaves on the mice liver. The study was used completely randomized design with 7 treatments and 6 replications.The mice was administrated the ethanol extract by dosages 250, 500 and 1000 mg/kg bw, whereas spinasterol dose were 26 and 52 mg/kg bw, and for control were used distilled water and DMSO. The treatment was given orally by gavage for 9 and 18 constitutive days. The morphological and histopathological parameters were observed at the end of experimental period. The observation of liver morphology showed no significantly difference between all treatment groups compared control, while the liver histopathological showed significantly difference between the all treatment groups compared to control (p<0.05), except for the ethanol extract by dosage 250 mg/kg bw. The highest score of liver histopathology was shown by ethanol extract of 1000 mg/kg body weight. The score was based on the degeneration of parenchyma, as well as karyolisis and necrosis of hepatocytes. It can be concluded that the highest dose of ethanol extract and spinasterol of senggugu leaves was toxic against the liver histological structure of mice.