Growth Optimization of Thermophilic Bacteria Bacillus thermoamylovorans and Brevibacillus sp. in Producing Keratinolytic Enzyme

Yohandini, Heni and Muharni, M. and Nainggolan, Eggy Lifrety (2015) Growth Optimization of Thermophilic Bacteria Bacillus thermoamylovorans and Brevibacillus sp. in Producing Keratinolytic Enzyme. In: The 5th International Seminar on New Paradigm and Innovation on Natural Sciences and Its Application (5th ISNPINSA) i , 7-8 Oktober 2015, Semarang.

[img]
Preview
PDF - Published Version
319Kb

Abstract

Some thermophilic bacteria have been isolated from Tanjung Sakti hot spring. Two isolates which qualitatively showed keratinolytic activity, identified as Bacillus thermoamylovorans and Brevibacillus sp., were optimized the growth in producing keratinase enzyme. Keratinase is a group of proteolytic enzyme which able to hydrolyze insoluble protein (keratin). In this research, whole chicken feathers were used as keratin substrate. The growth curves and measurement of keratinase enzyme activities were determined to the isolates, with a span of measuring 4 hours during 48 hours incubation, and gained the growth and optimum enzyme activity at 28 h incubation. The isolates were then observed the growth and keratinase activity at several different temperatures and pH. The results showed that the optimum temperature and pH for both isolates were same, i.e. at temperature 70° C and pH 7. The effect of adding a source of carbon and nitrogen into the medium were also observed. The growths of both isolates were increased on the addition of 1% glucose and 0.4% casein.

Item Type:Conference or Workshop Item (Paper)
Subjects:Q Science > QD Chemistry
Divisions:Faculty of Science and Mathematics > Department of Chemistry
ID Code:62396
Deposited By:Faculty of Engineering
Deposited On:16 May 2018 13:53
Last Modified:16 May 2018 13:53

Repository Staff Only: item control page