Utilization of Channels Digestion Golden Snail (Pomacea Canaliculata) as Lytic Enzyme and Application on Yeast Pichia Manshurica DUCC-Y15

Wijanarka, W. and Hidayat, Jafron W. and Parman, Sarjana (2015) Utilization of Channels Digestion Golden Snail (Pomacea Canaliculata) as Lytic Enzyme and Application on Yeast Pichia Manshurica DUCC-Y15. In: The 5th International Seminar on New Paradigm and Innovation on Natural Sciences and Its Application (5th ISNPINSA) i , 7-8 Oktober 2015, Semarang.

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Abstract

Mollusks were one of invertebrate animals that have not been studied and used in in the field of enzyme. One type of mollusk that has not been exploited significantly is golden snail (Pomacea canaliculata), especially in the digestive tract for the production of lytic enzymes. Lytic enzymes can be used as a microbial cell wall-breaking agents that will produce protoplasts, besides that this enzyme plays an important role in protoplast isolation technique producing good and healthy protoplasts. Protoplasts derived from the yeast Pichia manshurica DUCC-Y15is capable of producing the inulinase enzyme. The aim of this study was to use the digestive tract golden snail (Pomacea canaliculata) as lytic enzymes and to determine the amount of the released protoplasts at a concentration level of lytic enzymes different from the digestive tract golden snail (Pomacea canaliculata). Lytic enzyme concentrations used in this study was 75% (E3) and 100% (E4). The results showed that the digestive tract golden snail (Pomacea canaliculata) can produce lytic enzymes, the higher the concentration of lytic enzymes digestive tract golden snail given, then the higher the protoplasts were released. At a concentration of 75% lytic enzyme (E3) liberate protoplasts of 6.7 x 1017 (33.4%) and 100% (E4) of 9.9 x 1017 (45%).

Item Type:Conference or Workshop Item (Paper)
Subjects:Q Science > QD Chemistry
Divisions:Faculty of Science and Mathematics > Department of Chemistry
ID Code:62392
Deposited By:Faculty of Engineering
Deposited On:16 May 2018 13:34
Last Modified:16 May 2018 13:37

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