Utilization of Channels Digestion Golden Snail (Pomacea Canaliculata) as Lytic Enzyme and Application on Yeast Pichia Manshurica DUCC-Y15

Abstract

Mollusks were one of invertebrate animals that have not been studied and used in in the field of enzyme. One type of mollusk that has not been exploited significantly is golden snail (Pomacea canaliculata), especially in the digestive tract for the production of lytic enzymes. Lytic enzymes can be used as a microbial cell wall-breaking agents that will produce protoplasts, besides that this enzyme plays an important role in protoplast isolation technique producing good and healthy protoplasts. Protoplasts derived from the yeast Pichia manshurica DUCC-Y15is capable of producing the inulinase enzyme. The aim of this study was to use the digestive tract golden snail (Pomacea canaliculata) as lytic enzymes and to determine the amount of the released protoplasts at a concentration level of lytic enzymes different from the digestive tract golden snail (Pomacea canaliculata). Lytic enzyme concentrations used in this study was 75% (E3) and 100% (E4). The results showed that the digestive tract golden snail (Pomacea canaliculata) can produce lytic enzymes, the higher the concentration of lytic enzymes digestive tract golden snail given, then the higher the protoplasts were released. At a concentration of 75% lytic enzyme (E3) liberate protoplasts of 6.7 x 1017 (33.4%) and 100% (E4) of 9.9 x 1017 (45%).