METODE ANALISIS SPEKTOFOTOMETRI VISIBEL UNTUK MENETAPKAN KONSENTRASI POLIFENOL DALAM TEH HITAM PRODUKSI TAMBI (Method Of Visible Spectrophotometry Analysis for Determining of Polifenol Concentration in Tambi Black Production Tea)

Abstract

Polyphenol in tea or often referred to as catechins are different substances with catechins contained in other plants. Catechins in tea do not adversely affect the digestion of food. Tea catechins are antimicrobial (bacteria and viruses), antioxidants, antiradiate, strengthen blood vessels, launch secretions and inhibit cancer cell growth. Catechins are influential on all components of tea. In polyphenol removal using extraction method using 100oC Aquadest. The purpose of extraction is to withdraw the chemical components present in the material. This extraction is based on the principle of mass transfer of the component of the substance into the solvent, where the displacement begins at the interface layer and diffuses into the solvent. Spectrophotometer is a device used to measure relative energy if the energy is transmitted, reflected or emitted as a function of wavelength. In practice the concentration analysis of polyphenols using Visibel spectrophotometric instrument with 725nm wavelength. In making variable analysis of polyphenol concentration in black tea using Tambi production. Known black tea Tambi production contains a quite high concentration of polyphenols because black tea itself does contain high polyphenols second order after green tea. The result of polyphenol experiment of black tea samples obtained concentration 26.01 , 35.793, 56.089, 76.568, 94.649, 110.701, dan 128.782 (ppm). Keywords : Pholypenol,Extraction,SpectrophotometryVisible