PENGELOLAAN LIMBAH CAIR NON DAIRY CREAMER SEBAGAI MEDIA TUMBUH Saccharomyces cerevisiae UNTUK PRODUKSI PROTEIN SEL TUNGGAL DAN FUNGSI BIOREMEDIASI

Abstract

Limbah cair industri non dairy creamer dapat mencemari lingkungan bila tidak dilakukan pengolahan dengan baik. Bahan-bahan pencemar yang terkandung dalam limbah cair berupa senyawa organik dan anorganik baik dalam keadaan tersuspensi maupun terlarut mampu menurunkan mutu badan air bahkan menimbulkan bau sehingga dapat mengganggu keseimbangan lingkungan. Pemanfaatan limbah cair non dairy creamer sampai saat ini belum optimal, sehingga perlu suatu upaya untuk menjadikan limbah tersebut lebih berguna yaitu sebagai alternatif bahan baku produk lain dengan memanfaatkannya sebagai media tumbuh Saccharomyces cerevisiae untuk produksi protein sel tunggal, dan fungsi bioremediasi, agar lingkungan juga terjaga dari pencemaran oleh limbah cair non dairy creamer. Penelitian ini bertujuan menganalisis pengelolaan limbah cair non dairy creamer sebagai media tumbuh Saccharomyces cerevisiae untuk produksi protein sel tunggal dan fungsi bioremediasi. Penelitian lingkungan dilakukan di Dukuh Cabean, Kelurahan Mangunsari, Kecamatan Sidomukti Kota Salatiga. Alasan pemilihan lokasi berdasarkan letak industri non dairy creamer yaitu PT.Kievit yang ada di daerah tersebut, berikut lokasi pembuangan limbah yang berhubungan dengan badan air di sekitar pemukiman masyarakat. Penelitian ekperimental dilaksanakan di Laboratorium Kimia Fakultas Sains dan Matematika Universitas Kristen Satya Wacana, Salatiga. Hasil analisis lingkungan memperlihatkan bahwa pengolahan limbah cair non dairy creamer PT. Kievit Salatiga yang dialirkan di sepanjang aliran buangan limbah tidak menimbulkan pencemaran bagi lingkungan sekitar industri. Hasil analisis multivariat menunjukkan bahwa pemberian konsentrasi media 100%, 75%, 50% dan 25% tidak berpengaruh secara signifikan terhadap jumlah sel dan berat kering sel dengan (p > 0,05) namun berpengaruh secara signifikan terhadap kadar protein yang dihasilkan dengan nilai (p < 0,05). Dalam penelitian ini disimpulkan bahwa variasi konsentrasi substrat dan variasi konsentrasi sel Saccharomyces cerevisiae berpengaruh terhadap kadar protein yang dihasilkan. Produksi protein tertinggi dicapai pada konsentrasi media 75% dan konsentrasi sel 107 (sel/ml). Saccharomyces cerevisiae memiliki kemampuan bioremediasi terhadap senyawa organik terutama gliserol pada proses fermentasi media limbah cair non dairy creamer. Dari hasil penelitian direkomendasikan bahwa limbah cair non dairy creamer dapat dimanfaatkan menjadi bahan baku media tumbuh Saccharomyces cerevisiae, dan Saccharomyces cerevisiae dapat berperan dalam fungi bioremediasi senyawa organik. Kata Kunci : Limbah cair non dairy creamer, Saccharomyces cerevisiae, protein sel tunggal, bioremediasi Wastewater of non-dairy creamer industry can pollute the environment if not done properly processing. Pollutants contained in liquid waste in the form of organic compounds and anorganic either in a state of suspended and dissolved able to degrade the quality of water bodies pose even smell that can disrupt the environmental balance. Utilization of wastewater non-dairy creamer until now not optimal, so it needs to be an effort to make it more useful that waste as alternative raw material for another product by using it as a medium to grow Saccharomyces cerevisiae for the production of single cell protein, and bioremediation functions, so that the environment is also protected from contamination by wastewater non-dairy creamer. This study aims to analyze the management of liquid waste non dairy creamer Saccharomyces cerevisiae as a growing medium for the production of single cell protein and bioremediation functions. Environmental research conducted in Cabean Hamlet, Village Mangunsari, District Sidomukti Salatiga. The reason for choosing the location based on where the industry is PT.Kievit non-dairy creamer in the region, following disposal site waste associated with water bodies around human settlements. Experimental research conducted at the Laboratory of Chemistry, Faculty of Science and Mathematics Christian Satya Wacana University, Salatiga. Environmental analysis results showed that the wastewater treatment of non dairy creamer PT. Kievit Salatiga, which flowed along the flow of waste disposal does not cause pollution effects, for the environment industry. Multivariate analysis showed that the concentration of media 100 % , 75 % , 50 % and 25 % did not significantly effect on the number of cells and cell dry weight with (p > 0.05) but significantly on levels of a protein produced by the (p < 0 05) . In this study concluded that the variations in the concentration of the substrate and the variation of the concentration of Saccharomyces cerevisiae cells affect the levels of protein produced. The highest protein production was achieved in 75 % of media concentration and the concentration of 107 cells (cells/ml). Saccharomyces ceevisiae have the ability bioremediation towards organic compounds, especially glycerol in the fermentation process media wastewater non-dairy creamer. From the results of the study recommended that the wastewater non-dairy creamer can be used as raw material for growing media Saccharomyces cerevisiae and Saccharomyces cerevisiae can play a role in bioremediation function of organic compounds. Keywords: Wastewater of non-dairy creamer, Saccharomyces cerevisiae, single cell protein, bioremediation