AL-BAARRI, Ahmad N and LEGOWO, Anang M (2014) ENHANCED METHOD FOR AMPLIFYING ANTIMICROBIAL ACTIVITY OF LACTOPEROXIDASE SYSTEM IN MILK AND DERIVED PRODUCTS BY CARROT EXTRACT AND BETA CAROTENE. Technical Report. Undip, Semarang.
OSCN– has been known and well documented as natural antimicrobial agent generated from the lactoperoxidase system (LPOS) but the antimicrobial activity exerted from this system is too low for certain food such as milk and derived products (up to 1.2 log CFU/ml) resulting in the restriction on industrial development of LPOS. Our previous study concluded that involvement of carrot extract and beta carotene in LPOS significantly boosted the antimicrobial activity (up to 6 log CFU/ml) against S. enteritidis. This finding should be continued to the application on food. Since we found that LPOS generates low antimicrobial activity on milk and derived product, our research will be conducted on the application of LPOS plus carrot extract and beta carotene on milk and derived product. Because the high antimicrobial activity is needed for industrial purposes, this research may open the way for industrial development of natural antimicrobial agent from LPOS. This research will be conducted in three steps of experiment: (1) ensuring the incredible antimicrobial activity of LPOS plus carrot extract and beta carotene against three poisonous tropical bacteria: C. jejuni, S. enteritidis, and E. coli, (2) utilization of LPOS plus carrot extract and beta carotene in milk: full cream and skimmed milk, and (3) utilization of LPOS plus carrot extract and beta carotene in milk derived product: yogurt and tropical fruity milk. The purification of LPO from bovine milk, the complimentary data analysis for generation of LPOS antimicrobial activity by the addition of carrot extract and beta carotene will be conducted in Japan and for the application in tropical poisonous bacteria, milk, and milk derived product will be conducted in Indonesia. The outcomes of this research from three step of research are three international publications at international‐scopus‐indexed‐journals : International Journal of Dairy Science and one of patent: “enhanced method for improvement of LPOS antimicrobial activity by involvement of carrot extract and beta carotene in milk and derived product”. Keywords: Lactoperoxidase, antimicrobial activity, carrot extract, beta carotene, milk, derived products
|Monograph (Technical Report)
|S Agriculture > S Agriculture (General)
|Faculty of Animal and Agricultural Sciences > Department of Food Technology
|21 Jun 2017 14:05
|21 Jun 2017 14:05
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