Influence of field-based nutrient enrichment on the photobiology of the giant clam Tridacna maxima

Abstract

Nutrients were added to 12 microatolls in One Tree Island lagoon every low tide for 13 mo to an initial concentration of 10 lM (ammonium, N) and 2 lM (phosphate, P). These concentrations remained above background for 2 to 3 h after addition. The addition of ammonium (N and N+P but not P alone) significantly increased Pg (gross photosynthesis) Pn (net photosyn- thesis) and R (respiration) per unit wet-tissue weight and a (photosynthetic efficiency) in Tridacna maxima after 3 mo nutrient enrichment. These responses to small and transient changes in ammonium concentrations suggest that symbiotic clams are not nutrient-replete, and that even subtle changes in nutrients can have a measurable effect on photosynthesis. The same clams did not show significant differences in photosynthetic parameters 6 mo after the beginning of nutrient enrichment, suggesting that their previous responses had either been seasonal or that symbiotic clams such as T. maxima are able to adjust their photophysiology following external changes in nutrient concentrations.