EXPLORATION OF BACTERIA Symbionts Mangrove Waste FOR THE PRODUCTION OF DECOMPOSTER

Abstract

Mangrove waste comprises of naturally decomposed dead mangrove leaves, twigs, and branches. This research aims to determine the types of bacterial symbionts in mangrove waste with potency as anti-bacterial agents. These anti-bacterial agents will subsequently be used in the production of compost with bio-activators. The research process involves isolation of symbiont bacteria, identification for symbiont bacteria with potency as anti-bacterial agent, DNA extraction using High Pure PCR Temperature Preparation Kit (Roche), DNA amplification by PCR 16s rDNA, and DNA Sequencing. Resulting amplified 16S rDNA are analyzed and then sequenced using Genetix program. Symbionts with identified anti-bacterial properties are used in bio-activator production. Samples of Sargassum seaweed are treated separately with resulting bio-activator product from the research and other bio-activator products for nutritional content comparison. Four types of symbiont bacteria are identified as potential anti-bacterial agents, namely Pseudomonas sp., Flavobacterium sp., Acinetobacter sp., Bacillus subtilis. It is further found that bio-activator products from mangrove waste have better quality compared to those found in the market and non-bio activator added liquid organic fertilizers. Therefore, bio-activators from mangrove waste is a potential alternative as natural bio-activator products