PERBEDAAN KUANTITAS DNA YANG DIEKSTRAK DARI AKAR RAMBUT BERBAGAI FASE PERTUMBUHAN

Abstract

Background: Hair is a biological specimen that is often found in crime scene investigation. Forensic examination usually classify hair based on the acquisition method to estimate the quantity of deoxyribonucleic acid (DNA) obtained. There are no studies in Indonesia that had uncover the differences in extracted DNA quantity from each growth phases of the hair roots. Objective: To analyze the differences in the extracted DNA quantity from various hair root phases. Methods: The study was an observational study with cross sectional design using samples of hair roots (n = 15) that includes three phases of hair root growth, namely the anagen phase (n = 5), the catagen phase (n = 5), and the telogen phase (n = 5). The classification of the hair root phases is done through microscopic examination. All samples were extracted with Chelex, the DNA was quantified using NanoDrop spectrophotometer. The statistical test used is One-way Anova and Post Hoc Tamhane to see the differences between groups. Results: The average quantity of DNA extracted from each hair root phases are 28.78 ng/μl for anagen phase, 7.35 ng/μl for catagen phase, and 3.84 ng/μl for telogen phase. There were significant differences between groups of the hair roots phases (p <0,001). The significant differences were found between the anagen and catagen phases (p = 0,025), anagen and telogen phases (p = 0,015), and catagen and telogen phases (p = 0,014). Conclusions: There are significant differences in the extracted DNA quantity from various hair root phases. Keywords: DNA quantity, hair root phases, anagen, catagen, telogen