PENGARUH PEMBERIAN RANITIDIN TERHADAP GAMBARAN HISTOPATOLOGI PARU TIKUS WISTAR PADA PEMBERIAN METANOL DOSIS BERTINGKAT

Abstract

Background: Abuse of methanol toxicity poisoning in the body of the lungs and formic acidemia is a hazardous intoxication. The acidosis therapy for methanol poisoning is to suppress alcohol dehydrogenase (ADH) enzyme as an inhibitor to prevent formic accumulation. Ranitidine has gastric alcohol and hepatic aldehyde dehydrogenase enzymes as an inhibitor ativity. Aim: To prove the effect of ranitidine administration on lung hystopathology of the rats exposed by multilevel dose of methanol. Methods: True experimental post-test only with controlled group design. Samples were thirty five 2-3 months, 150-250 grams weight male wistar rats were divided into seven groups randomly: control group ( K ) was only given standard food and drink, positive control group 1 ( K1 ) was given a dose of ¼ LD- 100 methanol, positive control group 2 ( K2 ) was given a dose of ½ LD - 100 methanol, positive control group 3 ( K3 ) was given a dose of 1 LD - 100 methanol, treatment group 1 ( P1 ) was given ¼ LD - 100 methanol and 30 mg / kgBW dose of ranitidine, treatment group 2 ( P2 ) was given ½ LD – 100 methanol and 30 mg/kgBW dose of ranitidine and treatment group 3 ( P3 ) was given 1 LD - 100 methanol and 30 mg/kgBW dose of ranitidine. This research was implemented for 8 days. On the 8th day, the rats were terminated for examining the lung hystopathology. Results: To the positive control group and the treatment group were found the view of Histopathology alveolar edema, the destruction interalveolaris septum and infiltration of inflammatory cells from Mann Whitney dan Independent Sam- ples T-Test. Statistical test between groups of K2 and P2 oedema view and destruction indicates significant differences (p < 0.05), while K1 with P1 and K3 with P3 on oedema and destruction view also on infiltration view indicates a contrast result insignificant difference (p > 0.05). The observation between P1 , P2 , P3 showed heavier oedema microscopic, destruction and infiltration than K0, whereas it was statictically significant (p < 0.05) . Conclusion: Ranitidine administration wasn’t take effect on lung hystopathology of the rats exposed by multilevel dose of methanol. Keyword: Methanol, ranitidine, histopathology, lung.