PENGARUH PEMBERIAN INJEKSI KETOROLAC TROMETHAMINE INTRAPERITONEAL TERHADAP GAMBARAN MIKROSKOPIS GASTER TIKUS WISTAR DEWASA DENGAN FRAKTUR KRURIS

Abstract

Background: Diseases and abnormal conditions may cause severe pain, such as bone fracture. An accurate pain management is needed, in which opioid analgesic is the best choice. However, opioid is associated with addiction and side effects that affect the central nervous system. Therefore, ketorolac tromethamine, an NSAID, is used. However, NSAID has side effects that could cause a histopathological lesion in gaster. Objective: To investigate the effects of ketorolac tromethamine intraperitoneal injection on the histology of gaster in adult Wistar rats with crural fracture. Methods: An experimental study with post test only control group design. A sample of 20 Wistar rats divided into 2 groups, and had their right cruris fracturised. The 10 in control group are fed with standard food and water, while the 10 in intervention group are given ketorolac tromethamine 5mg/kgBB. Ketorolac tromethamine is given by intraperitoneal injection for 5 days at the start of the experiment. On the 8th day the rats are terminated with choloform overdose. The gasters were then harvested and made into microscopic sections with HE staining, and had their epithelial integrity evaluated with light microscope. Results: Mean gaster epithelial integrity score for the control group is 0.50+-0.170, while the score for the intervention group is 1.240+-0.158. Mann Whitney test showed a significant difference of p=0.00012 Conclusion: Ketorolac tromethamine intraperitoneal injections had effects on the histology of gaster in adult Wistar rats with crural fracture Key words: Fracture, Ketorolac tromethamine, Microscopic picture of stomach.