GAMBARAN HISTOPATOLOGI PADA SALURAN NAPAS BAWAH INTRAVITAL, PERIMORTEM DAN POSTMORTEM MENCIT BALB/C YANG DIBERI PAPARAN API

Abstract

Background: A burn is the most common injury occured in the world and it is a type of trauma which have high morbidity and mortality. A high incidence of burns become a challenge to the investigators, forensic experts, and law enforcement officers to distinguish wether the burns occured before death, around the time of death, or after death. Aim: to know the difference of intravital, perimortem and postmortem histopathological changes of lower respiratory tract in Balb/c mice with fire exposure. Methods: This study was an experimental study with Post Test-Only Control Group Design. Samples were 49 Balb/c mice that devided into 7 groups. Group K was a control (without fire exposure). Group P1 was given intravital fire exposure for 10 seconds. Group P2 was given intravital fire exposure for 20 seconds. Group P3 was given perimortal fire exposure for 10 seconds. Group P4 was given perimortal fire exposure for 20 seconds. Group P5 was given postmortal fire exposure for 10 seconds. Group P6 was given perimortal fire exposure for 20 seconds. After the intervension, the lungs were taken for microscopic. The data was analysed using Kruskal-Wallis and Mann-Whitney test. Results: Kruskal-Wallis test showed significant difference of all groups in vascular dilatation parameter (p bronchial=0,022; p bronchioles=0,010; p alveolar=0,000) and inflammatory cells parameter (p bronchial=0,005; p bronchioles=0,024; p alveolar=0,002). In soot parameter, there was significant different of bronchial and alveolar group (p bronchial=0,005; p alveolar=0,002), but in bronchioles group showed no significant difference (p bronchioles=0,709). Conclusions: There was significant difference of bronchial, bronchioles, and alveolar group in vascular dilatation and inflammatory cells parameter. In soot parameter, significant difference was showed in bronchial and alveolar group, but not in bronchiolus group. Keywords: burn, vascular dilatation, inflammatory cells, soot, lower respiratory tract