PENGARUH PEMBERIAN MINYAK JELANTAH TERHADAP PROFIL FARMAKOKINETIK PARASETAMOL PADA TIKUS WISTAR

Abstract

Background: The use of cooking oil that has been heated repeatedly are excessively done in the society to suppress expenses in cooking. Cooking oil that heated and used repeatedly could form a free radical that could saturate an endogenous antioxidant, namely glutathione. Paracetamol has analgesic and antipyretic effects which are widely used by the people. In the metabolism of paracetamol, glutathione have an important role. Objectives: To determine the effect of repeatedly heated oil on paracetamol pharmacokinetic in the Wistar rats’ blood. Methods: A true-experimental with post-test only control group design study using wistar as animal model. After adaptation, samples were randomly devided into two groups (n=7 pergroup). K (negative control), P was given repeatedly heated oli diet ad libitum for 56 days. On day 67th, all samples were given oral paracetamol 12,5 mg/200gBW. Blood sampling were done from retroorbita vein at 3, 5, 10, 20, 30, 40, 60, 90, 120, 180, 240, 300, and 360 minutes. Levels of paracetamol in plasma were measured by UV spectrophotometer at 242 nm. Results: Statistic test by independent t test showed significant differences in the parameters of Cl, Kel, t1/2e, Cmax, and AUC (p <0.05) and showed no significant differences in the parameters of Ka, Vd, and tmax (p> 0.05). Conclusion: Repeatedly heated oil could affect the paracetamol pharmacokinetic by increasing the t1/2e, Cmax, AUC and decreasing the Cl, and Kel Keywords: Repeatedly heated oil, paracetamol, pharmacokinetic parameters