PENGARUH PEMBERIAN RANITIDINE TERHADAP HISTOPATOLOGI HIPOKAMPUS TIKUS WISTAR DENGAN INTOKSIKASI METANOL AKUT

Abstract

Background: Methanol is one of the compounds that can cause systemic damage due to acidosis. One of the organs affected is the hippocampus. Ranitidine is known to reduce the levels of formic acid in the blood and as the consequence will reduce the toxic effects of methanol. Objective: To determine the effect of ranitidine on the hippocampus histopathology of acute methanol intoxicated Wistar rats. Methods: True experiment research design with parallel post test only control group design. Wistar strain rats aged 2-3 months (n = 15) were randomly divided into 3 groups (negative control group (n = 5), the positive control group (n = 5), and treatment (n = 5)). At 8 hours post-treatment, the mouse brains were taken and the percentage of necrosis hippocampal cells were examined with HE staining. Kruskal-Wallis and continued using Mann-Whitney statistical analysis were done to seek the difference between control and treatment groups. Results: In this study, there was an increase in the number of necrosis hippocampal cells in positive control group compared to the negative control group and a decrease in the treatment group compared to the positive control group. There were significant differences between the negative control group (p = 0.008), and treatment (p = 0.008) compared with the positive control group. However, there is no difference between the treatment groups compared to the negative control group (p = 0690). Conclusion: Intervention of ranitidine may decrease necrosis degree of hippocampal cells of Wistar rats with acute methanol intoxicated. Keywords: Ranitidine, hippocampus necrosis, acute methanol intoxication.