ANALISA KADAR LIKOPEN PADA TOMAT DENGAN MENGGUNAKAN SPEKTROFOTOMETER VISIBLE (Analysis content of lycopen on Tomato Using Spectrophotometer visible)

Abstract

Spectrophotometer is an instrument to measure or absorban that has the samples as a function of wavelength and measurement of a samples at a wavelength particular. One of the spectrophotometer working principle is based the phenomenon of absorption of light by certain species in the ultraviolet and visible light (visible). Raw materials used in this praktical is tomato. Tomato is a fruit that has high enough levels of lycopene. Lycopene including one group of carotenoid compounds. Therefore, carotenoids including terpenoids, then lycopene also include terpenoids. Lycopene is found in fruits, the giver of red color on the fruit. In this study was observed 5 variables: the ratio of sample versus solvent 1:1,1:2,1:3,1:4, and 1:5 and the variable temperature of 300C, 400C, 500C, 600C, and 700C. Produce variable levels of lycopene in the 0,770mg, 2,233mg, 3,004mg, 4,209mg and 4,782mg. Comparison of the solvent resulted in a higher lycopene.The things that affect the results of practicum is the cuvette hygiene as it can cause errors in the reading of absorbance and transmittance. Keywords: Spectrophotometer, Tomato, Lycopene