PENERAPAN METODE SPEKTROFOTOMETER SINAR TAMPAK UNTUK MENGUJI KANDUNGAN GLUKOSA PADA WORTEL (Daucus Carota L.) The Application of Visible Spectrophotometer Method for Testing of Glucose Content from Carrot (Daucus Carota L.)

Abstract

Spectrophotometry is one of the methods in chemical analysis needed to determine the composition of a sample both quantitatively and qualitatively based on the interaction between matter and light. Equipment used in spectrophotometry called a spectrophotometer. Carrot is a root vegetable plant species which are generally orange yellowish or reddish yellow with textures like wood, annuals life, up to 1 meter, can live well in cold regions, or plateau. On the practical determination of glucose in Carrots (Daucus Carota L.) with different variables obtained solvent concentration levels of 1.907% respectively; 1.926%; 1.940%; 1.978% and 1.990%, with a variable ratio of solvent extract and 1: 1, 1: 2, 1: 3, 1: 4 and 1: 5. It can be concluded that the more variables, the solvent used his much greater energy transfer so that the levels produced greater. In the lab variable 2 with a concentration of 100 ppm with a temperature of 50°C, 60°C, 70°C, 80°C and 90°C obtained levels of 1.997%; 1.960%; 1.94%; 1.930% and 1.900%. It can be concluded that the higher the temperature used, the maximum glucose extract that can be extracted. Kata kunci : Spectrophotometry, Carrot, Glucose