ANALISA AKTIVITAS ENZIM DIASTASE PADA MADU MENGGUNAKAN SPEKTROFOTOMETER SPECTONIC GENESYS 20 VISIBLE (Analysis Of Activities Diastase Enzyme In Honey Use Spectrophotometer Spectonic Genesys 20 Visible)

Abstract

Spectrophotometer is a tool used to measure the absorbance, concentration and transmittance. Measurements were made with the determination of the wavelength. One type of spectrophotometer is visible spectrophotometer, which uses visible light (Visible) as the light source and the wavelength of visible light is 380 to 750 nm. Visible light sources used are Tungsten lamps. This analyze uses honey as the main ingredient analyzer test diastase enzyme activity. The function of the diastase enzyme to decipher complex carbohydrates into simple carbohydrates.Iodinesolution 0.0007 N and a solution of starch solution is used as a standard solution. Absorbance measurements on samples to determine the linear equation to calculate the levels of the diastase enzyme done every interval of 5 minutes. Wavelength of 660 nm is used, absorbance and time graph will be used also to determine tx (The time is obtained from the determination of the value of the absorbance 0.235 of the sampling time) in the calculation Diastase Number (DN) using Schade and Codex Alimentariusmethods. Keywords: Spectrophotometer, Honey, Diastase Enzymes