ANALISA KADAR ASAM AMINO PADA DAUN PEPAYA DENGAN MENGGUNAKAN SPEKTROFOTOMETER VISIBLE (Analysis content of amino acid on papaya leaf using spectrophotometer visible)

Abstract

Spectrophotometer is an instrument for measuring the transmittance or absorbance of a sample as a function of wavelength and measurement of sample series at a particular wavelength. One spectrophotometer working principle is based on the phenomenon of light absorption by specific chemical species in the ultra violet and visible light (visible) .This material used raw in this lab is papaya. Papaya leaves are the leaves of papaya tree that has the amino acid level is quite high. Amino acid is one of the 20 most common types of monomers used in the formation of proteins. One function of amino acids is to help improve the process of ammonia detoxification by the liver. Amino acids can be found in fruits, vegetables and meat. In this study was observed 5 variables: the ratio of solvent extract with 10: 1, variable concentrations of 10-30 ppm standard solution and the wavelengths of 400-420 nm. Generate optimum levels of the amino acid is at a concentration of 15 with a wavelength of 420 nm. The things that affect the results of the lab is the cleanliness of the cuvette as it can cause errors in the reading of absorbance and transmittance. Keywords: Spectrophotometer, Papaya Leaf, Amino Acids