PENGARUH PEMBERIAN ASAM LEMAK TRANS TERHADAP MEDIATOR PROINFLAMASI, KADAR GLUKOSA DARAH DAN INFILTRASI NETROFIL PADA PULAU LANGERHANS

Abstract

Diabetes mellitus (DM) prevalence especially for type II is increasing rapidly world wide also in Indonesia, due to diet practice change, from previoulsy traditional food to become western food style and progressive high consumption of fast food. Fast food does not only contain high calory and fat but also abundant number of Trans fatty acid (TFA). TFA is known to be associated with profil lipid alteration which might result in cardiovascular disease eventually. This research was aimed to identify the changes of proinflamation mediator, blood glucose, and neutrofil infiltration in islets of Langerhans, pancreas. Thirty male Sprague dawley rats were divided into 3 treatment groups equally as follow; control, treatment group with 5% TFA diet (P1), and treatment group with 10% TFA diet (P2), initially baseline measurement for each variable was undertaken. In week -4 and -8 of observation period, a siginificant result was observed for hsCRP increasing value, with the highest increment percentage for week - 8 with 158%. Blood glucose level was significantly increased for both treatment groups P1 and P2, with more prominent value presented in P2 grop after 8 weeks. There was no neutrofil infiltration in control group, mild infiltration in group P1 and moderate infiltration in group P2. This research concluded negative effects of TFA intake in DM predictor panel. Key word: TFA (trans fatty acid), blood glucose level, proinflammatory mediator, neutrofil mediator