PENGARUH PEMBERIAN KOMBINASI 5FULEUCOVORIN DENGAN PHALERIA MACROCARPA TERHADAP PROLIFERASI SEL DAN DIAMETER ADENOKARSINOMA KOLON TIKUS SPRAGUE DAWLEY

Abstract

Introduction The incidence of colon cancer in the world is still high and the most cause of death of non-communicable disease category. Surgery remains the top choice therapy with other modalities such as chemotherapy, radiation and immunotherapy as P macrocarpa (Mahkota Dewa). This study seeks to prove the effects of fruit extracts P macrocarpa against colon adenocarsinoma, especially in terms of cell proliferation and changes in diameter of the tumor mass. Method Laboratory experimental research with post-test only design. This study used a female Sprague dawley white rats were divided into 4 groups : group K, P1 (chemotherapy group), P2 (P macrocarpa group), and P3 (combination group). Colon tumors induced by 1,2-DMH subcutaneously.Chemotherapy given 5FUleucovorin as much as 6 cycles according Rosswell Park Regiment. P macrocarpa given at a dose of 0,495 mg / day (0.99 mL / day) orally. Cell proliferation was assessed by IHC Ki-67 staining, while the diameter of the tumor mass was measured using a caliper tumor. Result Mean the cell proliferation and diameter of tumor masses on K, P1, P2, P3 group was 37.150 ± 8.878, 28.567 ± 12.531, 35.533 ± 8.982, 22.567 ± 3.445 and 1.403 ± 0.265, 1.135 ± 0.154, 1.339 ± 0.111, 1.074 ± 0.164 respectively. Statistical analysis showed a significant difference in cell proliferation between Kvs P3 (p=0.011), P2 vs P3 (p=0.022) and significant difference in diameter of the tumor mass between K vs P1 (p=0.020), K vs P3 (p=0.005), P2 vs P3 (0.021). Correlation analysis of cell proliferation with a diameter of the tumor mass was not significant (p=0.405). Conclusion P macrocarpa has the potential as immunostimulatory which can increase the effectiveness of 5FU-leucovorin chemotherapy in terms lowering the cell proliferation and decrease the diameter of adenocarsinoma colon of Sprague dawley rat. Keywords : P macrocarpa, colon adenocarsinoma, cell proliferation, tumor mass diameter